Composite

Part:BBa_K4345020:Design

Designed by: Elias Maris   Group: iGEM22_KU_Leuven   (2022-09-30)


5' UTR of hsp17 with ccdA fused to sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1149
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 569
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our team used the RNA thermometer as a part of a killswitch. The sequence was implemented before an antitoxin (ccdB) to regulate its translation based on temperature. It is possible to implement a part of the sequence of the gene that will be translated within the sequence that forms secondary hairpin structure. By doing this, leaky expression will be reduced. Because of the typical short sequence of this thermometer and the position of the startcodon, our team decided not to. The position of the startcodon can be found in the provided figure.


Source

Implemented in the mRNA structure of a cyanobacterial (Synechocystis sp.) heatshock gene which is important for stress tolerance.

References

Kortmann, J., Sczodrok, S., Rinnenthal, J., Schwalbe, H., & Narberhaus, F. (2010, December 3). Translation on demand by a simple RNA-based thermosensor. Nucleic Acids Research, 39(7), 2855–2868. https://doi.org/10.1093/nar/gkq1252